Coding

Part:BBa_K3711002:Design

Designed by: Jiacheng Shi   Group: iGEM21_HUST-China   (2021-10-01)


ROX1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1244
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 163
    Illegal SapI site found at 422


Design Notes

We optimized the codon of the gene to adapt to the yeast expression system.


Source

yeast


References